General Steps during HPLC Analysis

1. On the basis of individual application, select the types of HPLC.
2. Prepare the instrument for analysis.
3. Create Acquisition method in software.
4. Prepare the applicable system suitability solution and standard solution.
5. Create acquisition sequence and acquire the system suitability/standard solution.
6. Sample solution acquisition.
7. Process the chromatograms with suitable processing method.
8. Calculate and report the results.

Figure details the entire flow of activities involved in sample analysis through the utilization of HPLC.