In this method, the peak area of the analyte is expressed as a percentage of the total peak area of all components in the chromatogram, commonly referred to as %Area or Area %. The %Area represents the relative proportion of the analyte peak area compared to the total area of all peaks in the chromatogram.

ADVANTAGES

Simplified Data Analysis: The %Area method simplifies data analysis by expressing the analyte peak area as a percentage of the total peak area in the chromatogram.

Compensation for Injection Variations: The %Area method helps compensate for variations in injection volumes. Since the %Area is calculated based on the relative proportion of the analyte peak area to the total peak area, it can mitigate errors arising from differences in sample injection volumes.

Relative Quantitation: The %Area method enables relative quantitation, allowing for comparisons between samples without the need for absolute concentration values.

DISADVANTAGES

Assumption that Response Factor is the Same for All Peaks: The %Area method assumes that all components in the chromatogram have similar response factors.

Limited Accuracy: The %Area method may provide less accurate results compared to absolute quantitation methods.

Lack of Absolute Quantitation: The %Area method does not provide absolute concentration values for analytes. It can only offer relative measurements compared to the total peak area or other reference components, making it less suitable for applications requiring precise concentration determination.

The %Area or Area Normalization method is particularly useful when relative comparisons between samples or monitoring changes in analyte levels are the primary focus, rather than absolute quantification. Figure displays the steps involved in Area Normalization method.